Investigation of Some Pesticides’s Effects on Lipase Under In vitro Conditions

It is known that the enzyme activities of non-target organisms are influenced in vivo or vitro by pesticides play a major role to obtain higher yields field agriculture. foreseen used for various purposes may negatively affect lipase, an digestive system, non target organisms. Therefore, this study, effect diazinon (organophosphate class) and carbaryl (carbamate pesticides, which be widely agriculture, was investigated effective dosage range these on lipase determined. observed treated with concentration 100-2500 ppm inhibited. Lipase activity at maximum calculated as 2.05%. Carbaryl completely inhibited The Kinetics conformed Michaelis-Menten model Lineweaver-Burk graph drawn. showed strongest inhibitory against (Ki: 460.96±28.25 mM; IC50: 2.5±1.3 µM) comparison diazinon. In case diazinon, Ki IC50 value were found 481.32±45.18mM 3.6±0.9 µM respectively. results C. rugosa carbryl vitro. shown catalytic competitively but noncompetitively diazinon.Keywords: Lipase, Diazinon, Carbaryl, Pesticide, Inhibition kinetics